NISSOS adopts the new European AFQ Certification Mark of FoodOxys

The European AFQ certification mark of FoodOxys introduces a novel system for rating and classifying the quality of foods and beverages. Each product is subjected to a thorough
evaluation of its composition bioactivity to investigate its potential effects on human health and wellbeing.

According to the AFQ Certification Regulation, the 5 NISSOS beers were subjected to strict testing protocols in assessing their ability to neutralize a multitude of synthetic and natural
free radicals. Furthermore, their ability to prevent free radical-induced DNA damage, that can potentially lead to mutations, was also examined. Finally, the beers were tested for their
ability to protect important endogenous biomolecules, such as lipids, from oxidative damage, associated with modern lifestyle related cardiovascular diseases, neurological disorders, and
cancer.

NISSOS beers received particularly high scores in all assays tested. NISSOS GREEK ISLAND PILSNER was rated with 16/20, the ALL-DAY ORGANIC with 17/20 and the THOLI and the 7 BEAUFORT with 19/20. Finally, APOCALYPSE was rated with the highest possible score, 20/20, demonstrating the highest bioactivity in all biochemical protocolstested. Noteworthy, NISSOS beers seem to approach the bioactivity levels of red wine.

wine.

Among the 24 commercially available beers that were analyzed, the lowest score was 9/20,
while the average score was 12/20.

During the announcement of the AFQ certification for NISSOS beers, Professor of Physiology-
Toxicology of the University of Thessaly and Chairman of the Scientific Committee of

FoodOxys, Dr. Demetriοs Kouretas stated: "The biological value of beer for human health has
not been discussed, explained, and studied to the extent that wine has. Depending on the
production process and the raw materials used, beer may contain a wealth of antioxidants
that provide important benefits for human health. Phenols and melanoidins are the main
components contributing to the antioxidant properties of beer. At the same time, the low
ethanol content offers a very competitive advantage over wine, containing significantly higher
levels of alcohol. Antioxidants are important compounds that help us stay healthy by
counteracting the detrimental effects of oxidative stress. Clinical and preclinical studies that
have evaluated the protective effects of moderate beer consumption against cardiovascular
and other diseases demonstrate that the non-alcoholic compounds exert most of the
beneficial effects of beer (1, 2). The antioxidants contained in NISSOS beers reduce the free
radicals produced as by-products of human metabolism. Consequently, their moderate
consumption can exert a protective effect against various pathological conditions.".

Alexandros Kouris, Founder and Owner of NISSOS brewery, based in Tinos Island, Greece
stated: "Consumers, globally demand foods and beverages that benefit human health and
wellbeing. Today, NISSOS beers are the first to successfully comply with the demanding AFQ
Certification Regulation for high bioactivity. Although beer is the most popular alcoholic
beverage worldwide, consumer expectations from beer consumption are – unjustly – very
limited. FoodOxys AFQ proves that not all beers are the same. Overcoming stereotypes we
discover a new world of flavors, aromas, textures, colors, materials, techniques, but also great

potential benefits for human health of the moderate consumption of high-quality, "better-
for-you" beer! The high levels of antioxidants in NISSOS beers are derived from the quality

raw materials used and from the brewing process applied. The potent endogenous antioxidant
activity of the unpasteurized and preservative-free NISSOS beers protects their taste during
the time between production and consumption and exerts beneficial effects on human health.
The antioxidant profile of our beers affects not only their shelf life, but also the stability of
their taste, as well as protecting them from oxidations that could potentially reduce their
quality and benefits (3). Therefore, the antioxidants contained in NISSOS beers, are the most
important endogenous "guardians" of their taste and the "guarantors" of their beneficial
effects on human health.".
FoodOxys (www.foodoxys.com) is a spin-off company of the University of Thessaly, Greece
which is active in the study of nutritional wellness. Its scientific team consists of distinguished
researchers with many years of experience in the field of biochemistry of nutrition and human
metabolism. Under the scientific guidance of Professor Demetrios Kouretas, FoodOxys
scientists have published more than 230 papers in world-renowned scientific journals and
have considerable experience in the biochemistry of nutrition.
The AFQ (https://www.foodoxys.com/afq/τι-είναι-το-afq/). The European certification mark
AFQ introduces a novel system for rating and classifying agri-food products from around the
world based on the properties mentioned in its Certification Regulation.
NISSOS (https://nissos.beer) is a family owned, independent, multi-awarded brewery located
on the Cycladic Island of Tinos in Greece. NISSOS beers are slow brewed, 100% natural, using
only carefully selected malts, aromatic hops, and yeast. They have rich taste and aroma,
natural carbonation, and maximum nutritional value. Our beers do not undergo heat
treatment (pasteurization), do not contain preservatives, and are only stabilized by
microfiltration and/or their endogenous antioxidants.